INTERACTIVE EFFECTS OF SALINITY AND NATURAL NITROGF;N SOURCE ON SOME METABOLIC ACTIVITIES OF CHLORELLA VULGARIS

INTERACTIVE EFFECTS OF SALINITY AND NATURAL NITROGF;N SOURCE
ON SOME METABOLIC ACTIVITIES OF CHLORELLA VULGARIS
SHAALAN, S. H.; KHALEAFA, A. F. AND EL-SAID, A. A. F.
Department of Botany and Microbiology, Faculty of Science,
Alexandria University.
ABSTRACT
Chlorella vulgaris (Chlorophyceae) was cultured on media
of combined mixtures containing different salinity levels
(ranging from 50·200 mM NaCl) and cow waste extract of
concentrations ranging from 0.2·1.4 %0. Bold’s basal medium
was used as a control. The interactive effects of salinity
and natural nitrogen source of the test media on the
intracellular accumulation of some organic and inorganic
solutes were investigated.
The obtained results revealed that the reducing sugars,
total free amino acids and free proline content increased
under the increasing salinity levels. while they showed a
reverse trend with rise in waste concentration. On the other
hand, the protein content together with both nitrate and
ammonia contents decreased with the increased levels of
external salinity. However, increasing waste concentrations
were paralleled with remarkable intracellular accumulations
of proteins, nitrate and ammonia under an yof the
investigated salinity levels.