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vol-31IMMUNOSUPPRESSIVE EFFECTS OF METRIFONATE ON
OREOCHROMIS NILOTICUS
EL-GOHARY, M .S
1
., SAFINAZ, G. MOHAMED
2
, KHALIL , R.H
3
EL- BANNA. S
4
AND SOLIMAN, M.K
3
1Animal Health Research Institute , Kafr El Sheikh .
2
Institute of Ochanography and Fisheries , Alex . Branch .
3Dept . of Avian Aquatic Med . Fac . of Vet . Med . Alex. Univ.
4Dept. Aquatic Aimal Med. Agric.Rec.Center, Animal Health Res. Inst. Alex. Laboratory
Key words: Immunosuppressive, Metrifonate, Oreochromis niloticus.
ABSTRACT
The effect of metrifonate on the immunity of the exposed fish are evaluated through the
determination of the antibody titer and mortality rate during the challenge trial. In the
chronic toxic exposure to metrifonate by dose 0.17mg/liter for 8 weeks, the more clear
signs were darkness of fish color with hemorrhagic patches & loosing of scale at end of the
trial the color return to normal. Determination of some blood parameters revealed
significant decrease of white blood cell count, as there were lymphopenia and nueutrophilia
during the chronic exposure to metrifonate. Phagocytic assay revealed a significant
decrease in both phagocytic activity (PA) and phagocytic index(PI).Hypoproteinami
hypoglobulinaemia and hypoalbuminaemia were observed. The challenge trial indicted
that, the metrifonate decrease the immunity of exposed fish.. The using of biogen
“R”
as a
growth promoter in fish diet minimized the toxicity of metrifonate.
