GROWTH PERFORMANCE AND ELECTROPHORETIC PATTERN OF F1 AND F2 INJECTED OREOCHROMIS NILOTICUS WITH CHICKEN DNA

GROWTH PERFORMANCE AND ELECTROPHORETIC
PATTERN OF F1 AND F2 INJECTED OREOCHROMIS
NILOTICUS WITH CHICKEN DNA
EL-DEEB, S.I.,* EZZAT, A.** AND HELMY, Z.A.*
*National Institute of Oceanography and Fisheries, Alexandria.
**Faculty of Science Alexandria University
Key words: Transgenesis, DNA, injection, Oreochromis niloticus.
ABSTRACT
In the present investigation we tried transfer chicken DNA to Oreochromis
niloticus and evaluate its effect on the growth performance and the electrophoretic pattern
of sarcoplasmic protein. To achieve this purpose, mature males and females received two
concentrations of fractionated chicken DNA (10µ and 20µ) injected in intraperitoneal
cavity on two times through one week.
The first generation of injected parents (F1
IP) were fed with practical diets
containing different protein levels (20%, 25%, 30%, 35% and 40%) for 178 days to
evaluate the feeding level which produce the best growth rate. The growth performance and
electrophoretic pattern of the F1
IP and F2
IP and chicken were studied.
It’s clear that:
1- the growth performance increased in both F1
IP and F2
IP compared with
control
2-the electrophoretic patterns of sarcoplasmic proteins of the studied groups
showed presence from (15 to 17) fractions in control fish; from (15 to 19)
fractions in both F1
IP at different protein levels in diet; (17) fractions in both
F2
IP and (20) fractions in chicken.
Fractions (7, 17 and 29) appeared in chicken and in two generations of injected parents
and this refer to gene transfer occurred.